|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5132476||1378814||2018||5 صفحه PDF||سفارش دهید||دانلود کنید|
- Gluten free claim compliance at the serving-size level provides the best protection.
- Gluten free oats under Purity Protocol found 4% non-compliant at serving-size level.
- Non-homogenous grinding dictates a reliable way to assess gluten compliance in oats.
- Analytical scheme proposed and verified to assess gluten in gluten-free oats.
A step-wise, 'test-all-positive-gluten' analytical methodology has been developed and verified to assess kernel-based gluten contamination (i.e., wheat, barley and rye kernels) during gluten-free (GF) oat production. It targets GF claim compliance at the serving-size level (of a pouch or approximately 40-50Â g). Oat groats are collected from GF oat production following a robust attribute-based sampling plan then split into 75-g subsamples, and ground. R-Biopharm R5 sandwich ELISA R7001 is used for analysis of all the first15-g portions of the ground sample. A >20-ppm result disqualifies the production lot, while a >5 to <20-ppm result triggers complete analysis of the remaining 60-g of ground sample, analyzed in 15-g portions. If all five 15-g test results are <20Â ppm, and their average is <10.67Â ppm (since a 20-ppm contaminant in 40Â g of oats would dilute to 10.67Â ppm in 75-g), the lot is passed.
Journal: Food Chemistry - Volume 240, 1 February 2018, Pages 391-395