کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5133055 1378823 2018 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of a simple, low cost chronoamperometric assay for fructose based on a commercial graphite-nanoparticle modified screen-printed carbon electrode
چکیده انگلیسی


- A chronoamperometric assay was successfully developed for fructose in fruit juice.
- Graphite nanoparticles on a screen-printed electrode improved electron transfer.
- Sensitivity & precision of the measurement were enhanced for fructose.
- Fructose was successfully determined in a commercial fruit juice product.
- Only a simple dilution step, prior to analysis, was required.

This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies.Calibration studies were carried out using chronoamperometry with a 40 µl mixture containing fructose, mediator and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1 mM to 1.0 mM. A commercial fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477 mM with a precision of 3.03% (n = 5). Following fortification (477 mM fructose) the mean recovery was found to be 97.12% with a coefficient of variation of 6.42% (n = 5); consequently, the method holds promise for the analysis of commercial fruit juices.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 241, 15 February 2018, Pages 122-126
نویسندگان
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