|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5133055||1492051||2018||5 صفحه PDF||سفارش دهید||دانلود کنید|
- A chronoamperometric assay was successfully developed for fructose in fruit juice.
- Graphite nanoparticles on a screen-printed electrode improved electron transfer.
- Sensitivity & precision of the measurement were enhanced for fructose.
- Fructose was successfully determined in a commercial fruit juice product.
- Only a simple dilution step, prior to analysis, was required.
This paper describes the development of a simple, low cost chronoamperometric assay, for the measurement of fructose, using a graphite-nanoparticle modified screen-printed electrode (SPCE-G-COOH). Cyclic voltammetry showed that the response of the SPCE-G-COOH enhanced the sensitivity and precision, towards the enzymatically generated ferrocyanide species, over a plain SPCE; therefore the former was employed in subsequent studies.Calibration studies were carried out using chronoamperometry with a 40Â Âµl mixture containing fructose, mediator and FDH, deposited onto the SPCE-G-COOH. The response was linear from 0.1Â mM to 1.0Â mM. A commercial fruit juice sample was analysed using the developed assay and the fructose concentration was calculated to be 477Â mM with a precision of 3.03% (nÂ =Â 5). Following fortification (477Â mM fructose) the mean recovery was found to be 97.12% with a coefficient of variation of 6.42% (nÂ =Â 5); consequently, the method holds promise for the analysis of commercial fruit juices.
Journal: Food Chemistry - Volume 241, 15 February 2018, Pages 122-126