کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5134074 1492074 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Betalains increase vitexin-2-O-xyloside cytotoxicity in CaCo-2 cancer cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Betalains increase vitexin-2-O-xyloside cytotoxicity in CaCo-2 cancer cells
چکیده انگلیسی


- Vitexin-2-O-xyloside was purified from Beta vulgaris cicla seeds.
- Betalains were purified from red beet root by anionic exchange chromatography.
- Betalains increase vitexin-2-O-xyloside cytotoxicity in CaCo-2 colon cancer cells.
- The cytotoxic effect is mediated by activation of an intrinsic apoptotic pathway.

Vitexin-2-O-xyloside (XVX) from Beta vulgaris var. cicla L. (BVc) seeds, betaxanthin (R1) and betacyanin (R2) fractions from Beta vulgaris var. rubra L. (BVr) roots were combined and tested for cytotoxicity in CaCo-2 colon cancer cells. XVX was the most cytotoxic molecule, but the combination of XVX with R1 and R2 significantly prolonged its cytotoxicity. Cytotoxicity was mediated by the intrinsic apoptotic pathway, as shown by an increase in Bcl2-like protein 4, cleaved Poly ADP-Ribosyl Polymerase 1 and cleaved Caspase 3 levels with a parallel decrease in anti-apoptotic protein B-cell leukemia/lymphoma 2 levels. R1 and R2, used alone or in combination, reduced oxidative stress triggered by H2O2 in CaCo-2 cells. Betalains dampened cyclooxygenase-2 and interleukin-8 mRNA expression after lipopolysaccharide induction in CaCo-2, showing an anti-inflammatory action. Our results support the use of a cocktail of R1, R2 and XVX as a chemopreventive tool against colon cancer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 218, 1 March 2017, Pages 356-364
نویسندگان
, , , , , , , , ,