کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5530840 1401769 2017 6 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen
چکیده انگلیسی


- We evaluate the effect of addition of quercetin in an extender containing DMA or glycerol on freezability of goat semen.
- Supplementation of extender with 10 μM quercetin in combination with DMA improves the goat sperm motion kinetics.
- Supplementation of extender with 5% of DMA and 10 μM may improve motility, membrane integrity and functionality.
- The DMA is more effective cryoprotectant for freezing of the goat.

This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was pooled and extended by egg yolk based extender supplemented with different concentrations of quercetin (10 or 20 μM) along with 5% glycerol or DMA. The extended semen was frozen and sperm motility parameters, viability, abnormality, membrane integrity and lipid peroxidation were assessed after thawing. Results showed that sperm viability, total motility, progressive motility, straightness (STR) and linearity (LIN) were higher (P < 0.05), and abnormality percentage and MDA concentration were lower (P < 0.05) in extender containing DMA. Similarly, higher (P < 0.05) total motility, progressive motility, viability and membrane integrity along with lower (P < 0.05) MDA level were noted in Q10 group. The lowest (P < 0.05) MDA level was observed in DMA extender containing moderate level of quercetin (Q10D). Also the STR was higher (P < 0.05) in Q10D compared to Q10G and Q20G groups. In conclusion, supplementation of extender with 10 μM quercetin in combination with DMA improves the goat sperm motion kinetics and suppresses lipid peroxidation after freezing and thawing. Furthermore, DMA is more effective cryoprotectant for the freezing of goat sperm.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 75, April 2017, Pages 15-20
نویسندگان
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