کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7639314 | 1494820 | 2016 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lithium attenuates lead induced toxicity on mouse non-adherent bone marrow cells
ترجمه فارسی عنوان
لیتیم باعث کاهش سمیت ناشی از سرب در سلول های مغز استخوان غیر سلول می شود
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کلمات کلیدی
PBSDMEMH3K9acSDSHMGBhistone H3 lysine 9 acetylationPARPTCAFCSPAGELithium - لیتیمDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoMTT - MTTEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدtrichloroacetic acid - اسید ترشکلراکتیکpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدApoptosis - خزان یاختهایSOD - سدsodium dodecyl sulfate - سدیم دودسیل سولفاتLead - سربfetal calf serum - سرم گوساله جنینSuperoxide dismutase - سوکسوکس دیسموتازColony-stimulating factor - فاکتور تحریک کننده Colonyphosphate buffer saline - فسفات بافر شورCSF - مایع مغزی نخاعیPropidium iodide - پروتئین یدیدpoly ADP-ribose polymerase - پلی ADA-ribose پلیمراز
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Lead is a poisonous heavy metal that occurs in all parts of environment and causes serious health problems in humans. The aim of the present study was to investigate the possible protective effect of lithium against lead nitrate induced toxicity in non-adherent bone marrow stem cells. Trypan blue and MTT assays represented that exposure of the cells to different concentrations of lead nitrate decreased viability in a dose dependent manner, whereas, pretreatment of the cells with lithium protected the cells against lead toxicity. Lead reduced the number and differentiation status of bone marrow-derived precursors when cultured in the presence of colony stimulating factor (CSF), while the effect was attenuated by lithium. The cells treated with lead nitrate exhibited cell shrinkage, DNA fragmentation, anion superoxide production, but lithium prevented lead action. Moreover, apoptotic indexes such as PARP cleavage and release of HMGB1 induced by lead, were protected by lithium, suggesting anti-apoptotic effect of lithium. Immunoblot analysis of histone H3K9 acetylation indicated that lithium overcame lead effect on acetylation. In conclusion, lithium efficiently reduces lead toxicity suggesting new insight into lithium action which may contribute to increased cell survival. It also provides a potentially new therapeutic strategy for lithium and a cost-effective approach to minimize destructive effects of lead on bone marrow stem cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Trace Elements in Medicine and Biology - Volume 36, July 2016, Pages 7-15
Journal: Journal of Trace Elements in Medicine and Biology - Volume 36, July 2016, Pages 7-15
نویسندگان
Mahsan Banijamali, Azra Rabbani-Chadegani, Maryam Shahhoseini,