کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7640587 | 1494854 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid detection of hydrogen sulfide produced by pathogenic bacteria in focused growth media using SHS-MCC-GC-IMS
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A new rapid method for the detection of hydrogen sulfide from pathogenic bacteria is reported. The developed method, static headspace - multi-capillary column - gas chromatography - ion mobility spectrometry (SHS-MCC-GC-IMS), has been applied to detect hydrogen sulfide evolution from 61 bacteria. The developed method has been compared against a standard triple sugar iron (TSI) agar approach, and a modified single sugar iron (SSI) agar approach. Hydrogen sulfide detection by SHS-MCC-GC-IMS using an initial inoculum of 1-1.5â¯Ãâ¯105â¯CFU/mL can be achieved within 6â¯h, after incubation at 37â¯Â°C, with a limit of detection of 1.6â¯ng/mL. Data for the standard agar method against the new instrumental approach, and the modified agar method against the new instrumental approach, are compared. The specificity for the new method compared against the standard method and the modified agar approach across all 61 strains was 85.2% and 88.5% respectively, and 86.7% and 91.3% across the 23 Salmonella strains tested.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 140, July 2018, Pages 232-240
Journal: Microchemical Journal - Volume 140, July 2018, Pages 232-240
نویسندگان
Ryan Thompson, John D. Perry, Stephen P. Stanforth, John R. Dean,