کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8358252 | 1542064 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RNA silencing is induced by the expression of foreign recombinant products in transgenic rice
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PTGSOryza sativa L.DCLdsRNARNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکdouble stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNATGs - TG هاArgonaute - آرگونوتSDS-polyacrylamide gel electrophoresis - الکتروفورز ژل SDS-polyacrylamide gelSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدtransgene - ترانس ژنRNA silencing - خاموش شدن RNApost-transcriptional gene silencing - خاموش کردن ژن post-transcriptionaltranscriptional gene silencing - خاموش کردن ژن رونویسیRISC - خطرCo-suppression - سرکوب مشترکendoplasmic reticulum - شبکه آندوپلاسمی RNA-Induced Silencing Complex - مجتمع خاموش کننده RNA inducedUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهAGO - پیش
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
RNA silencing plays important roles in the regulation of gene expression in eukaryotes. We previously reported that RNA silencing of a linked endogenous gene and a transgene in transgenic rice seeds can be induced by the expression of foreign recombinant mGLP-1, which acts as a silencing-inducible sequence through RNA interference. In this study, we found that the induction of RNA silencing by foreign transgenes is not restricted to mGLP-1 but is observed in many other genes as a relatively general phenomenon, as several foreign genes were involved in inducing RNA silencing in the same manner as mGLP-1 in transgenic rice. We detected 21-24-nt siRNAs using both sense and antisense probes specific to the silenced genes in both the leaves and endosperm of transgenic rice plants. Moreover, read-through transcripts were consistently observed in silenced transgenic rice plants. Taken together, these results suggest that proper transcription termination was prevented in these plants, and the highly divergent 3â²-end transcripts served as templates for double-stranded RNA synthesis, resulting in the degradation of the target genes via siRNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 225, August 2014, Pages 138-146
Journal: Plant Science - Volume 225, August 2014, Pages 138-146
نویسندگان
Lijun Yang, Taiji Kawakatsu, Yuhya Wakasa, Masato Yoine, Fumio Takaiwa,