کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8546280 | 1561725 | 2018 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells
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کلمات کلیدی
AR42J cellsCCK-8sarcoendoplasmic reticulum Ca2+-ATPaseBAPNACM-H2DCFDATMRMSOD2EGTAGSHThapsigarginGSSGFCCPTPsFURA-2/AM - 2-فورا / AM5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate, acetyl ester - 5- (و -6) -کلرومتیل-2 '، 7'-دی کرولدید هیدروفلوورسین دی سکته، استیل استرcholecystokinin octapeptide - cholecystokinin oktapeptideROS - ROS[Ca2+]i - [Ca2 +] iHydrogen peroxide - آب اکسیژنهFura-2 acetoxymethyl ester - اتوکسی متیل استر فورا 2Sulfanilic acid - اسید سولفانیلیکSuperoxide dismutase 2 - سوکسوکس دیسموتاز 2endoplasmic reticulum - شبکه آندوپلاسمی intracellular free Ca2+ concentration - غلظت Ca2 + آزاد داخل سلولیSERCA - قلبtetramethylrhodamine methyl ester - متیل استر تترامتیل رودامینH2O2 - هیدروژن پراکسیدPancreas - پانکراسreduced glutathione - کاهش گلوتاتیونcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazoneCalcium - کلسیمoxidized glutathione - گلوتاتیون اکسید شدهReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We studied the effects of the tartrazine-metabolite sulfanilic acid on the physiology of pancreatic AR42J cells. Sulfanilic acid (1 μM-1 mM) induced a slow and progressive increase in intracellular free-calcium concentration that reached a plateau. The effect of sulfanilic acid was not concentration-dependent. Stimulation of cells with thapsigargin (1â¯Î¼M) after treatment with sulfanilic acid (1â¯mM) induced a smaller Ca2+ response compared with that obtained with thapsigargin alone. Sulfanilic acid induced a concentration-dependent production of reactive oxygen species; however, this effect was not Ca2+-dependent. Depolarization of mitochondrial membrane potential was observed at the concentration of 1â¯mM sulfanilic acid. In the presence of the compound a decrease in the GSH/GSSG ratio was observed. A decrease in the expression of superoxide dismutase 2 was noted. Finally, stimulation of cells with CCK-8 led to a concentration-dependent increase of trypsin secretion that was impaired by pretreatment of cells with sulfanilic acid. Preincubation of cells with the antioxidant melatonin (100â¯Î¼M) reduced the effect of sulfanilic acid on trypsin secretion. We conclude that sulfanilic acid might induce oxidative stress, which could alter Ca2+ signaling and enzyme secretion in pancreatic AR42J cells. This creates a situation potentially leading to damage of the exocrine pancreas.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 120, October 2018, Pages 71-80
Journal: Food and Chemical Toxicology - Volume 120, October 2018, Pages 71-80
نویسندگان
Fatma Zohra Ameur, Nabila Mehedi, Omar Kheroua, Djamel Saïdi, Gines M. Salido, Antonio Gonzalez,