کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749710 | 1050294 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of ER stress on unfolded protein responses, cell survival, and viral replication in primary effusion lymphoma
ترجمه فارسی عنوان
اثرات استرس ادرار بر پاسخ های پروتئینی باز نشده، بقای سلولی و تکرار ویروسی در لنفوم افسوفی اولیه
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کلمات کلیدی
Inositol-requiring enzyme-1CCAAT/enhancer-binding protein-homologous proteinprotein kinase-like ER kinaseUPRIRE1GADD34XBP1LANAThapsigarginATF6GRP78KSHVIRE1α - IRE1aPEL - از PELER stress - استرس استCHOP - تکه کردنX-box binding protein-1 - جعبه اتصال پروتئین -1endoplasmic reticulum - شبکه آندوپلاسمی activating transcription factor-6 - فعال کردن عامل رونویسی 6Primary effusion lymphoma - لنفوم اپوژن اولیهKaposi's sarcoma-associated herpesvirus - ویروسهای مرتبط با سارکوم کاپوزیUnfolded protein response - پاسخ پروتئین آشکار78 kDa glucose-regulated protein - پروتئین تنظیم شده با گلوکز 78 کیلو دیPERK - پرک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Primary effusion lymphoma (PEL), a subtype of non-Hodgkin's B-lymphoma, is an aggressive neoplasm caused by Kaposi's sarcoma-associated herpesvirus (KSHV) in immunosuppressed patients. Endoplasmic reticulum (ER) stress induces activation of the unfolded protein response (UPR), which induces expression of ER chaperones, which in turn decrease ER stress, leading to ER homeostasis. The UPR is necessary for not only ER homeostasis but also persistent infection by, and replication of, many viruses. However, the precise roles and regulation of the UPR in KSHV infection remain poorly understood. Here, we found that IRE1α and PERK were significantly downregulated in PEL cells cultured under normal conditions, compared with KSHV-uninfected B-lymphoma cells. IRE1α and PERK mRNA levels were decreased in PEL cells, and KSHV-encoded LANA and v-cyclin D led to suppressed IRE1α transcription. Thapsigargin-induced ER stress activated the UPR and increased the mRNA levels of UPR-related molecules, including IRE1α and PERK, in PEL cells. However the IRE1α and PERK mRNA levels in PEL cells were lower than those in KSHV-uninfected cells. Furthermore, ER stress induced by brefeldin A and thapsigargin dramatically reduced the viability of PEL cells, compared with KSHV-uninfected cells, and induced apoptosis of PEL cells via the pro-apoptotic UPR through expression of CHOP and activation of caspase-9. In addition to the pro-apoptotic UPR, thapsigargin-induced ER stress enhanced transcription of lytic genes, including RTA, K-bZIP and K8.1, and viral production in PEL cells resulted in induction of the lytic cycle. Thus, we demonstrated downregulation of IRE1α and PERK in PEL cells, transcriptional suppression of IRE1α by LANA and v-cyclin D, apoptosis induction in PEL cells by ER stress, and potentiation of lytic replication by ER stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 3, 15 January 2016, Pages 565-572
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 3, 15 January 2016, Pages 565-572
نویسندگان
Zenpei Shigemi, Yusuke Baba, Naoko Hara, Jumpei Matsuhiro, Hiroki Kagawa, Tadashi Watanabe, Masahiro Fujimuro,