کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11023206 1701328 2018 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection and quantification of ricin-mediated 28S ribosomal depurination by digital droplet PCR
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Detection and quantification of ricin-mediated 28S ribosomal depurination by digital droplet PCR
چکیده انگلیسی
Ricin acts to damage cells by producing a site of depurination in 28S ribosomal RNA. This depurination results in ribosome inactivation which inhibits protein synthesis and ultimately leads to cell death. We have developed a multiplexed digital droplet polymerase chain reaction assay that enables the objective measurement of toxin activity through quantitation of depurinated 28S rRNA molecules. This assay demonstrates the first use of digital PCR technology to measure ribotoxin-mediated damage. Depurination events were detected in ricin-treated lung cell cultures as early as 1 h, and within 9 h of exposure the maximum ribosomal damage of 70% was reached and was sustained for at least 24 h post-exposure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 563, 15 December 2018, Pages 15-19
نویسندگان
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