کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
19963 43150 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene
ترجمه فارسی عنوان
تجزیه و تحلیل ژن ژنوم گسترده سلول های بنیادی جنینی موش در معرض p-dichlorobenzene
کلمات کلیدی
غیر اختصاصی RNA؛ سلول های بنیادی جنینی موش؛ تعریف عمیق؛ p-Dichlorobenzene؛ ارزیابی سم شناسی
Non-coding RNA; Mouse embryonic stem cells; Deep sequencing; p-Dichlorobenzene; Toxicological assessment
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• We exposed mESCs to p-dichlorobenzene 1 or 28 days (daily dose).
• Mechanisms modulating proteins occurred following acute and chronic exposures.
• LncRNAs are novel biomarkers of the chemical stress response in mESCs.

Because of the limitations of whole animal testing approaches for toxicological assessment, new cell-based assay systems have been widely studied. In this study, we focused on two biological products for toxicological assessment: mouse embryonic stem cells (mESCs) and long noncoding RNAs (lncRNAs). mESCs possess the abilities of self-renewal and differentiation into multiple cell types. LlncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to chemicals. We exposed mESCs to p-dichlorobenzene (p-DCB) for 1 or 28 days (daily dose), extracted total RNA, and performed deep sequencing analyses. The genome-wide gene expression analysis indicated that mechanisms modulating proteins occurred following acute and chronic exposures, and mechanisms modulating genomic DNA occurred following chronic exposure. Moreover, our results indicate that three novel lncRNAs (Snora41, Gm19947, and Scarna3a) in mESCs respond to p-DCB exposure. We propose that these lncRNAs have the potential to be surrogate indicators of p-DCB responses in mESCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 3, September 2016, Pages 329–333
نویسندگان
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