کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
19969 43150 2016 6 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Calorimetric studies of the growth of anaerobic microbes
ترجمه فارسی عنوان
مطالعات کالوریمتریک رشد میکروب های بی هوازی
کلمات کلیدی
کالریمتری؛ Clostridium acetobutilicum؛ Clostridium beijerinckii؛ Clostridium cellulovorans؛ میکروب های بی هوازی؛ فرایندهای رشد
Calorimetry; Clostridium acetobutylicum; Clostridium beijerinckii; Clostridium cellulovorans; Anaerobic microbes; Growth processes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

This article aims to validate the use of calorimetry to measure the growth of anaerobic microbes. It has been difficult to monitor the growth of strict anaerobes while maintaining optimal growth conditions. Traditionally, optical density and ATP concentration are usually used as measures of the growth of anaerobic microbes. However, to take these measurements it is necessary to extract an aliquot of the culture, which can be difficult while maintaining anaerobic conditions. In this study, calorimetry was used to continuously and nondestructively measure the heat generated by the growth of anaerobic microbes as a function of time. Clostridium acetobutylicum, Clostridium beijerinckii, and Clostridium cellulovorans were used as representative anaerobic microbes. Using a multiplex isothermal calorimeter, we observed that peak time (tp) of C. acetobutylicum heat evolution increased as the inoculation rate decreased. This strong correlation between the inoculation rate and tp showed that it was possible to measure the growth rate of anaerobic microbes by calorimetry. Overall, our results showed that there is a very good correlation between heat evolution and optical density/ATP concentration, validating the use of the method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 3, September 2016, Pages 364–369
نویسندگان
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