کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
19973 43151 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Improvement of ethanol production by recombinant expression of pyruvate decarboxylase in the white-rot fungus Phanerochaete sordida YK-624
ترجمه فارسی عنوان
بهبود تولید اتانول توسط بیان نوترکیب دکربوکسیلاز پیروات در Phanerochaete sordida YK-624 قارچ پوسیدگی سفید
کلمات کلیدی
Phanerochaete sordida YK-624؛ قارچ سفید پوسیدگی؛ دیربوکسیلاز پیروات؛ بیان نوترکیب ؛ تولید اتانول
Phanerochaete sordida YK-624; White-rot fungi; Pyruvate decarboxylase; Recombinant expression; Ethanol production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Recombinant PDC gene was stably expressed by GP7.
• PDC gene expression of GP7 improved PDC productivity and ethanol production.
• The direct production of ethanol from wood was improved by using GP7.

To improve ethanol production by Phanerochaete sordida YK-624, the pyruvate decarboxylase (PDC) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. We screened 16 transformant P. sordida YK-624 strains that each expressed a second, recombinant PDC gene (pdc) and then identified the transformant strain (designated GP7) with the highest ethanol production. Direct ethanol production from hardwood was 1.41 higher with GP7 than with wild-type P. sordida YK-624. RT-PCR analysis indicated that the increased PDC activity was caused by elevated recombinant pdc expression. Taken together, these results suggested that ethanol production by P. sordida YK-624 can be improved by the stable expression of an additional, recombinant pdc.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 1, July 2016, Pages 17–21
نویسندگان
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