Chemical composition, antioxidant and antimicrobial activity of guavirova (Campomanesia xanthocarpa Berg) seed extracts obtained by supercritical CO2 and compressed n-butane

• Guavirova seeds extracts were produced using supercritical CO2 and compressed n-butane, with a high yield verified for n-butane (∼25 wt%).
• Antioxidant and antimicrobial activity of the extracts of C. xanthocarpa Berg seeds from CO2 and n-butane are reported.
• Pressurized n-butane can be more efficient in terms of extraction yield than supercritical CO2.

This work reports the chemical characterization, antioxidant and antimicrobial activity of Campomanesia xanthocarpa seed extracts obtained from compressed n-butane and supercritical CO2 extraction. For supercritical CO2, the extraction condition was 40 °C and 250 bar, while for n-butane 35 °C and 10 bar. The total phenolic compound content was 68.58 ± 1.15 mg g−1 for n-butane extract and 17.18 ± 2.44 mg g−1 for supercritical CO2. Both extracts were rich in terpenoids, especially β-caryophyllene, while n-butane extract had a larger content of flavonoids than supercritical CO2. The antioxidant activity was tested with FRAP, DPPH and deoxyribose assay, with n-butane extracts exhibiting better results. Antimicrobial activity was verified for both extracts against Pseudomonas aeruginosa, however, supercritical CO2 extract afforded a better performance, reaching 5.0 mg mL−1 for MIC and 10.0 mg mL−1 for MBC, while n-butane extract showed 40.0 mg mL−1 in both antimicrobial tests. Higher extraction yields were obtained using n-butane, which also presented higher antioxidant activity, while supercritical CO2 extracts afforded the highest antimicrobial activity.

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