کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5131535 1491316 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The photostability of the commonly used biotin-4-fluorescein probe
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The photostability of the commonly used biotin-4-fluorescein probe
چکیده انگلیسی

Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined number of biotin binding sites. Analytical testing showed that the biotin moiety in the probe undergoes a photosensitized oxidation to produce a mixture of biotin sulfoxides which has the potential to impact the quantitation of binding sites using this fluorescent probe.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 531, 15 August 2017, Pages 78-82
نویسندگان
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