کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5131549 | 1491313 | 2017 | 9 صفحه PDF | دانلود رایگان |
In this study, we developed a screen-printed carbonâgraphene-based electrochemical biosensor for EN2 protein detection. The engrailed-2 (EN2) protein, a biomarker for prostate cancer, is known to be a strong binder to a specific DNA sequence (5â²-TAATTA-3â²) to regulate transcription. To take advantage of this intrinsic property, aptamer probes with TAATTA sequence was immobilized onto the screen-printed carbonâgraphene electrode surface via EDCâNHS coupling approach. Cyclic voltammetry (CV) of the electrochemical measurement technique was employed for the quantitative detection of EN2 protein. The hindrance to the redox reaction of potassium ferricyanide on the biosensor surface due to the binding of the immobilized aptamer with its target EN2 protein quantified the protein concentration. Under optimum conditions, the aptamer biosensor can detect EN2 protein over a linear range from 35 to 185Â nM with a detection limit of 38.5Â nM.
Journal: Analytical Biochemistry - Volume 534, 1 October 2017, Pages 99-107