|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5131675||1491319||2017||4 صفحه PDF||سفارش دهید||دانلود کنید|
Rapid diagnostic tests can be developed using ELISA for detection of diseases in emergency conditions. Conventional ELISA takes 1-2 days, making it unsuitable for rapid diagnostics. Here, we report the effect of reagents mixing via shaking or vortexing on the assay timing of ELISA. A 48-min protocol of ELISA involving 12-min incubations with reagent mixing at 750Â rpm for every step was optimized. Contrary to this, time-optimized control ELISA performed without mixing produced similar results in 8Â h, leaving a time gain of 7Â h using the developed protocol. Collectively, the findings suggest the development of ELISA-based rapid diagnostics.
Journal: Analytical Biochemistry - Volume 528, 1 July 2017, Pages 53-56