|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5131675||1378764||2017||4 صفحه PDF||ندارد||دانلود کنید|
Rapid diagnostic tests can be developed using ELISA for detection of diseases in emergency conditions. Conventional ELISA takes 1â2 days, making it unsuitable for rapid diagnostics. Here, we report the effect of reagents mixing via shaking or vortexing on the assay timing of ELISA. A 48-min protocol of ELISA involving 12-min incubations with reagent mixing at 750Â rpm for every step was optimized. Contrary to this, time-optimized control ELISA performed without mixing produced similar results in 8Â h, leaving a time gain of 7Â h using the developed protocol. Collectively, the findings suggest the development of ELISA-based rapid diagnostics.
Journal: Analytical Biochemistry - Volume 528, 1 July 2017, Pages 53-56