کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5131806 1378773 2017 5 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide gel
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide gel
چکیده انگلیسی

A ring-shaped electroeluter (RSE) was designed for protein recovery from polyacrylamide gel matrix. The RSE was designed in such a way that a ring-shaped well was used to place gel slices and an enrichment well was used to collect eluted protein samples. With HSA as model protein, the electroelution time was less than 30 min with 80% recovery rate, and the concentration of recovered protein was 50 times higher than that of conventional method. The RSE could be reused at least ten times. The developed device makes great advance towards economic electroelution of biomolecules (such as proteins) from gel matrix.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 523, 15 April 2017, Pages 39-43
نویسندگان
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