کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5132619 | 1492050 | 2018 | 11 صفحه PDF | دانلود رایگان |
- Plant extracts induced apoptosis and arrested colon cancer cells in G1 phase.
- Black lentil, sorghum, red grape had the highest inhibition on colon cancer cells.
- Phenolic concentration strongly correlated with HT-29 and HCT 116 inhibition.
- Delphinidin-3-O-glucoside concentration strongly correlated with HT-29 inhibition.
- Cyanidin-3-O-glucoside was the most potent on tyrosine kinase inhibition.
The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r = 0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r = 0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9-2.0 mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (â8.5 kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50 = 0.10 and 2.37 µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.
Journal: Food Chemistry - Volume 242, 1 March 2018, Pages 378-388