کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5133101 1492051 2018 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Beaming steviol glycoside analysis into the next dimension
ترجمه فارسی عنوان
تجزیه و تحلیل استیوول گلیکوزید به ابعاد بعدی
کلمات کلیدی
Rebaudioside A؛ Rebaudioside D؛ استیوول گلیکوزید؛ Stevioside؛ کروماتوگرافی مایع با کارایی فوق العاده بالا؛ Rebaudioside A (PubChem CID: 6918840)؛ Rebaudioside C (PubChem CID: 60208888)؛ Rebaudioside D (PubChem CID: 71773169)؛ Rebaudioside F (PubChem CI
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- First ultra-high performance liquid chromatography application separating nine steviol glycosides.
- Nine UHPLC columns tested.
- Three columns yield resolution of 2 or greater for stevioside and rebaudioside A.
- Sufficient retention of rebaudioside D on two columns.
- Total run time 16 min.

Nine state-of-the-art reversed phase (RP) columns for ultra-high performance liquid chromatography were tested for the separation of steviol glycosides. The main criteria were resolution of the critical peak pair rebaudioside A and stevioside and the retention time of rebaudioside D. Three columns yielded a resolution of 2 or more of the critical peak pair and two of them showed sufficient retention of rebaudioside D, namely 1.62 and 1.84 min corresponding to retention factors of 0.98 and 1.24. The separation of nine steviol glycosides was possible in 11 min with UV and MS compatible, buffer-free eluents at moderate temperature. The presented method is proposed to be adopted as a new official method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 241, 15 February 2018, Pages 150-153
نویسندگان
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