کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5134120 | 1492073 | 2017 | 6 صفحه PDF | دانلود رایگان |
- A combination of two-state mechanism for trimethoprim and sulfamethoxazole determination was proposed.
- The sensitivity could be improved 301 and 329 times for SMZ and TMP, respectively.
- The limits of detection (LODs) were 8.5Â ng/mL for TMP and 7.7Â ng/mL for SMZ.
- The LODs were lower than the maximum residue limits in foods of animal origin.
An on-line preconcentration method combining micelle to solvent stacking (MSS) with field-amplified sample stacking (FASS) was employed for the analysis of trimethoprim (TMP) and sulfamethoxazole (SMZ) by capillary zone electrophoresis (CZE). The optimized experimental conditions were as followings: (1) sample matrix, 10.0Â mM SDS-5% (v/v) methanol; (2) trapping solution (TS), 35Â mM H3PO4-60% acetonitrile (CH3CN); (3) running buffer, 30Â mM Na2HPO4 (pHÂ =Â 7.3); (4) sample solution volume, 168Â nL; TS volume, 168Â nL; and (5) 9Â kV voltage, 214Â nm UV detection. Under the optimized conditions, the limits of detection (LODs) for SMZ and TMP were 7.7 and 8.5Â ng/mL, and they were 301 and 329 times better compared to a typical injection, respectively. The contents of TMP and SMZ in animal foodstuffs such as dairy products, eggs and honey were analyzed, too. Recoveries of 80-104% were acquired with relative standard deviations of 0.5-5.4%.
Journal: Food Chemistry - Volume 219, 15 March 2017, Pages 7-12