کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5137085 1494528 2017 11 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Neoagarooligosaccharides enhance the level and efficiency of LDL receptor and improve cholesterol homeostasis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Neoagarooligosaccharides enhance the level and efficiency of LDL receptor and improve cholesterol homeostasis
چکیده انگلیسی


- We investigated cholesterol-lowering effect of Neoagarooligosaccharide.
- Neoagarooligosaccharide improves lipid profiling in mice fed a high-cholesterol diet.
- Neoagarooligosaccharide increased LDL receptor via SREBP-2 activation.
- Neoagarooligosaccharide-induced LDL receptor expression is mediated by PI3K/Akt.
- Neoagarooligosaccharide decreased PCSK9 expression through HNF-1α reduction.

Neoagarooligosaccharides (NAOS) are produced by β-agarases through the cleavage of β-1,4 linkage in agarose. Here, we investigated the cholesterol-lowering properties of NAOS and its underlying molecular mechanism. Administration of NAOS in mice fed a high-cholesterol diet (HCD) inhibited hepatic fat accumulation and serum LDL cholesterol. Mice fed an HCD showed a decrease in LDL receptor (LDLR) expression, which was reversed by NAOS. Moreover, NAOS enhanced LDLR expression and LDL uptake in hepatocytes. NAOS increased nuclear translocation of SREBP-2, a crucial transcription factor for LDLR gene expression. Increased LDLR luciferase activity, induced by NAOS, was abolished on deletion of SRE in the LDLR promoter. NAOS upregulated LDLR levels via PI3K/Akt-mediated activation of SREBP-2. Furthermore, we demonstrated that NAOS reduced the nuclear HNF-1α, leading to decreased levels of PCSK9, a natural inhibitor of LDLR. These findings indicate that NAOS exhibits hypolipidemic activity, and may serve as a new hypolipidemic drug.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 38, Part A, November 2017, Pages 529-539
نویسندگان
, , , , , , , ,