کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5511275 | 1539852 | 2017 | 55 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Methylglyoxal upregulates Dictyostelium discoideum slug migration by triggering glutathione reductase and methylglyoxal reductase activity
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کلمات کلیدی
NACEASCd-Erythroascorbic acidEapx1D-arabinono-1,4-lactone oxidasereverse transcriptase quantitative PCRBSON-acetyl-l-cysteineMBBRRT-qPCRADH1GSHDTTORFASCODScAMP - cAMPDMSO - DMSOγ-glutamylcysteine synthetase - γ-گلوتامیل سستئین سینتاتازAscorbic acid - آسکوربیک اسیدalcohol dehydrogenase 1 - الکل دهیدروژناز 1thioredoxin reductase - تریودوکسین ردوکتازdithiothreitol - دیتیوتریتولDimethyl sulfoxide - دیمتیل سولفواکسیدSuperoxide dismutase - سوکسوکس دیسموتازOctadecyl silica - سیلیکا اکتادیدیلopen reading frame - قاب خواندن بازMethylglyoxal - متیل گلی اکسالMonobromobimane - مونوبرموبیمنCatalase - کاتالازhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراGlutathione - گلوتاتیونglutathione reductase - گلوتاتیون ردوکتازglutathione synthetase - گلوتاتیون سنتتازGlutaredoxins - گلوتاراکسین هاReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glutathione (GSH)-deprived Dictyostelium discoideum accumulates methylglyoxal (MG) and reactive oxygen species (ROS) during vegetative growth. However, the reciprocal effects of the production and regulation of these metabolites on differentiation and cell motility are unclear. Based on the inhibitory effects of γ-glutamylcysteine synthetase (gcsA) disruption and GSH reductase (gsr) overexpression on aggregation and culmination, respectively, we overexpressed GSH-related genes encoding superoxide dismutase (Sod2), catalase (CatA), and Gcs, in D. discoideum. Wild-type KAx3 and gcsA-overexpressing (gcsAOE) slugs maintained GSH levels at levels of approximately 2.1-fold less than the reference GSH synthetase-overexpressing mutant; their GSH levels did not correlate with slug migration ability. Through prolonged KAx3 migration by treatment with MG and H2O2, we found that MG increased after the mound stage in this strain, with a 2.6-fold increase compared to early developmental stages; in contrast, ROS were maintained at high levels throughout development. While the migration-defective sod2- and catA-overexpressing mutant slugs (sod2OE and catAOE) decreased ROS levels by 50% and 53%, respectively, these slugs showed moderately decreased MG levels (36.2 ± 5.8 and 40.7 ± 1.6 nmol gâ1 cells wet weight, P < 0.05) compared to the parental strain (54.2 ± 3.5 nmol gâ1). Importantly, defects in the migration of gcsAOE slugs decreased MG considerably (13.8 ± 4.2 nmol gâ1, P < 0.01) along with a slight decrease in ROS. In contrast to the increase observed in migrating sod2OE and catAOE slugs by treatment with MG and H2O2, the migration of gcsAOE slugs appeared unaffected. This behavior was caused by MG-triggered Gsr and NADPH-linked aldolase reductase activity, suggesting that GSH biosynthesis in gcsAOE slugs is specifically used for MG-scavenging activity. This is the first report showing that MG upregulates slug migration via MG-scavenging-mediated differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 90, September 2017, Pages 81-92
Journal: The International Journal of Biochemistry & Cell Biology - Volume 90, September 2017, Pages 81-92
نویسندگان
Hyang-Mi Lee, Ji-Hui Seo, Min-Kyu Kwak, Sa-Ouk Kang,