Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide

- Activity of Lipozyme 435 decreased with an increase of pressure, exposure time and temperature of SC-CO2.
- FT-IR analyses suggested a change in the secondary structure of the immobilized lipase.
- FESEM images did not present morphological alterations on the macroporous anionic resin (support).
- High yields and esterification rates of oleic acid under SC-CO2 were obtained.
- Esterification percentage in supercritical CO2 was 65% higher than in n-hexane medium.