Continuous enzymatic production of biodiesel from virgin and waste sunflower oil in supercritical carbon dioxide

▶ ScCO2-based continuous enzymatic conversion of sunflower oil into biodiesel. ▶ A two-stage fractionation process afforded FAME with >96% purity. ▶ Combination of Lipozyme + Novozym led to FAME yields nearing 99% for waste cooking oil.