کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7558187 | 1491364 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of 3-phenoxybenzoic acid in river water with a colloidal gold-based lateral flow immunoassay
ترجمه فارسی عنوان
تشخیص اسید 3-فینوکسی بنزوئیک در آب رودخانه با روش ایمونویسی جریان جانبی مبتنی بر طلا
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کلمات کلیدی
اسید 3-فینوسی سدیم اسید، ایمونواسی جریان جانبی جانبی، آنتیبادیهای مونوکلونال، طلا کلوئیدی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
3-Phenoxybenzoic acid (3-PBA) is a general metabolite of synthetic pyrethroids. It could be used as a generic biomarker for multiple pyrethroids exposure for human or pyrethroid residues in the environment. In this study, monoclonal antibodies (mAbs) against 3-PBA were developed by using PBA-bovine serum albumin (BSA) as an immunogen. In the competitive enzyme-linked immunosorbent assay (ELISA) format, the I50 and I10 values of purified mAbs were 0.63 and 0.13 μg/ml, respectively, with a dynamic range between 0.19 and 2.04 μg/ml. Then, the colloidal gold (CG)-based lateral flow immunoassay was established based on the mAbs. The working concentration of coating antigen and CG-labeled antibodies and the blocking effects were investigated to get optimal assay performance. The cutoff value for the assay was 1 μg/ml 3-PBA, and the detection time was within 10 min. A total of 40 river water samples were spiked with 3-PBA at different levels and determined by the lateral flow immunoassay without any sample pretreatments. The negative false rate was 2.5%, and no positive false results were observed at these levels. This lateral flow immunoassay has the potential to be an on-site screening method for monitoring 3-PBA or pyrethroid residues in environmental samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 483, 15 August 2015, Pages 7-11
Journal: Analytical Biochemistry - Volume 483, 15 August 2015, Pages 7-11
نویسندگان
Yuan Liu, Aihua Wu, Jing Hu, Manman Lin, Mengtang Wen, Xiao Zhang, Chongxin Xu, Xiaodan Hu, Jianfeng Zhong, Lingxia Jiao, Yajing Xie, Cunzhen Zhang, Xiangyang Yu, Ying Liang, Xianjin Liu,