کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7559404 1491399 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of plant nucleotide sugars by hydrophilic interaction liquid chromatography and tandem mass spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل قندهای نوکلئوتید گیاهی با استفاده از کروماتوگرافی مایع با تعامل هیدرولیکی و طیف سنجی جرم دو طرفه
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Understanding the intricate metabolic processes involved in plant cell wall biosynthesis is limited by difficulties in performing sensitive quantification of many involved compounds. Hydrophilic interaction liquid chromatography is a useful technique for the analysis of hydrophilic metabolites from complex biological extracts and forms the basis of this method to quantify plant cell wall precursors. A zwitterionic silica-based stationary phase has been used to separate hydrophilic nucleotide sugars involved in cell wall biosynthesis from milligram amounts of leaf tissue. A tandem mass spectrometry operating in selected reaction monitoring mode was used to quantify nucleotide sugars. This method was highly repeatable and quantified 12 nucleotide sugars at low femtomole quantities, with linear responses up to four orders of magnitude to several 100 pmol. The method was also successfully applied to the analysis of purified leaf extracts from two model plant species with variations in their cell wall sugar compositions and indicated significant differences in the levels of 6 out of 12 nucleotide sugars. The plant nucleotide sugar extraction procedure was demonstrated to have good recovery rates with minimal matrix effects. The approach results in a significant improvement in sensitivity when applied to plant samples over currently employed techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 448, 1 March 2014, Pages 14-22
نویسندگان
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