کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
816556 1469264 2016 7 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Inhibitory effect of aqueous extract of different parts of Gossypium herbaceum on key enzymes linked with type 2 diabetes and oxidative stress in rat pancreas in vitro
ترجمه فارسی عنوان
اثر مهاری عصاره آبی عصاره های مختلف گیاه گاسپیوم بر روی آنزیم های کلیدی مرتبط با دیابت نوع 2 و استرس اکسیداتیو در پانکراس موش آزمایشگاهی
کلمات کلیدی
گیاه پنبه، دیابت، مالون دی آلدئید، α-آمیلاز، α-گلوکوزیداز
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی (عمومی)
چکیده انگلیسی

This study sought to determine the inhibitory effect of aqueous extract of different parts (bark, leaf, and flower) of cotton plant (Gossypium herbaceum) on key enzymes linked with type 2 diabetes and oxidative stress in rat pancreas in vitro. The aqueous extract (1:10 w/v) of Gossypium herbaceum was prepared and the ability of the extract to inhibit the activity of α-amylase and α-glucosidase as well as activities of pro-oxidant Fe2+-induced lipid peroxidation was determined spectrophotometrically. The results revealed that the three varieties were able to inhibit the activity of α-amylase and α-glucosidase in rat's pancreas in a dose dependent manner (0–88.8 mg/ml). Also, the incubation of pancreas tissue homogenate in the presence of Fe2+ caused a significant increase (233.3%) in the malondialdehyde (MDA) content of pancreas homogenate, nevertheless, the introduction of the aqueous extract inhibited MDA production dose dependently (0–33.33 mg/ml) and also exhibited further antioxidant properties as represented by their high radical scavenging and Fe2+ chelating abilities. Inhibition of α-amylase and α-glucosidase activities has been the primary treatment for the management/prevention of type 2 diabetes. Therefore, the α-amylase and α-glucosidase inhibitory activities of aqueous extracts of different parts of Gossypium herbaceum in rat pancreas and prevention of lipid peroxidation in the tissue may be attributed to the presence of polyphenol content of the plant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Beni-Suef University Journal of Basic and Applied Sciences - Volume 5, Issue 2, June 2016, Pages 180–186
نویسندگان
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