کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9746470 | 1492160 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of high-pressure processing on proteolytic enzymes and proteins in cold-smoked salmon during refrigerated storage
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present paper describes the effects of high-pressure processing on the activity of proteolytic enzymes in cold-smoked salmon and enzyme extracts for pressures up to 300 MPa. The activities of the three enzymes, cathepsin B-like, cathepsin B + L-like and calpains were reduced at all pressure levels of up to 300 MPa (at ca. 9 °C for 20 min) in crude enzyme extracts prepared from cold-smoked salmon. Calpain almost completely inactivated at 300 MPa. High-pressure did not influence general proteolytic activity but activated the enzymes in muscles at higher pressure levels studied until 18 days of storage. An increase in the activity of cathepsin B + L-like and calpain was seen after 12 days of refrigerated storage. Myosin heavy chain was less affected at higher pressure levels (300 MPa) only as shown by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins and further refrigerated storage had no obvious effects on proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 90, Issue 4, May 2005, Pages 541-548
Journal: Food Chemistry - Volume 90, Issue 4, May 2005, Pages 541-548
نویسندگان
R. Lakshmanan, Margaret F. Patterson, John R. Piggott,