Microbiological, physicochemical, and sensory characteristics of kefir during storage

Changes in certain microbiological, physicochemical, and sensory parameters of kefir were studied during refrigerated storage. Kefir batches were prepared using 1% and 5% added kefir grains, and samples for analysis were taken 24 h after inoculation and then after 2, 7, 14, 21, and 28 days of storage at 5 ± 1 °C. After fermentation for 24 h after inoculation, lactobacilli and lactococci were present at levels of 108 cfu/ml, and yeasts and acetic acid bacteria were present at levels of 105 and 106 cfu/ml, respectively. The lactic acid flora decreased by about 1.5 log units between days 7 and 14 and then stabilized at that level. Yeast and acetic acid bacterial counts, lactose, and pH all remained constant over the storage period, while the total fat content and dry matter decreased. The percentage inoculate did exert an influence, and the sample batches made using 1% added kefir grains had higher lactic acid bacterial counts, lactose, and pH, while the sample batches made using 5% added kefir grains had higher yeast and acetic acid bacterial counts and viscosity. The total fat and dry matter contents were similar in both sample batches. Sensory analysis of the kefir samples revealed maximum acceptability levels in the first 2 days of storage.