Production and characterization of Escherichia coli glycerol dehydrogenase as a tool for glycerol recycling

► Cost-effective production of recombinant Escherichia coli glycerol dehydrogenase. ► Easy procedure for purification of Escherichia coli glycerol dehydrogenase. ► Wide enzyme kinetic characterization of glycerol oxidation reaction. ► Enzymatic conversion of glycerol from biodiesel waste into dihydroxyacetone.