کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
20027 43153 2016 5 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Quantitative analysis of protein orientation in membrane environments by kinase activity
ترجمه فارسی عنوان
تجزیه و تحلیل کمی از جهت گیری پروتئین در محیط غشا با فعالیت کیناز
کلمات کلیدی
AgrC؛ جهت گیری پروتئین؛ لیپوزوم؛ واکنش دهنده Thiol؛ فعالیت کیناز
AgrC; Protein orientation; Liposome; Thiol-reactive reagent; Kinase activity
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی

AgrC is an integral membrane receptor protein with histidine kinase activity in the accessory gene regulator (agr) quorum-sensing system of Staphylococcus aureus. In this study, proteoliposomes were used as a model to investigate AgrC orientation. Many approaches have been described to determine membrane protein orientation, but they are often complicated and time consuming. In this study, AgrC orientation in liposomes was determined by thiol-reactive reagent labeling and a kinase activity assay. Our results suggest use of a kinase activity assay could get an accurate percentage of functional protein orientation and only cost nearly one-sixth of the time compared with the method based on thiol-reactive reagent labeling. We present an effective and rapid method for determining the orientation of membrane protein kinases like AgrC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 2, February 2016, Pages 242–246
نویسندگان
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