کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
20111 43158 2016 6 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Purification and characterization of solvent tolerant lipase from Bacillus sp. for methyl ester production from algal oil
ترجمه فارسی عنوان
پاکسازی و مشخص کردن لیپاز تحمل کننده حلال از Bacillus sp. برای تولید متیل استر از روغن جلبک
کلمات کلیدی
Bacillus sp .؛ تحریم؛ تصفیه؛ حلال لیپاز مقاوم؛ Transesterification
Bacillus sp.; Immobilization; Purification; Solvent tolerant lipase; Transesterification
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Isolation of solvent tolerant lipase from Bacillus sp.
• Efficiency of isolated lipase activity with wide pH and temperature range.
• Satisfactory lipase activity towards the solvents and metal ions.
• Good operational stability of immobilized lipase.
• Isolated enzyme showed high transesterification activity.

Lipase from Bacillus sp. isolated from the oil contaminated soil was purified by ammonium sulphate precipitation and ion-exchange chromatography with a 5.1-fold purification and 10.5% yield. SDS-PAGE analysis of the enzyme revealed the molecular mass of 24 kDa. The optimum pH and temperature for lipase activity were 6.5 and 37°C, respectively. The isolated lipase was stimulated by pretreatment with methanol and ethanol as well as by divalent metal ions Ca2+, Mg2+ and Mn2+. The enzyme showed high activity towards oleic rich oils. The enzyme immobilized on celite could retain 90% lipase activity after eight cycles. Transesterification of Botryococcus sp. oil using the immobilized enzyme for 40 h resulted in 80% yield of fatty acid methyl esters which had good properties for use as biodiesel. Overall results suggested that the solvent tolerant Bacillus lipase can be a potential biocatalyst for methyl ester production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 5, May 2016, Pages 517–522
نویسندگان
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