کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
20133 43159 2016 4 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Lentiavidins: Novel avidin-like proteins with low isoelectric points from shiitake mushroom (Lentinula edodes)
ترجمه فارسی عنوان
Lentiavidins: پروتئین های آویدین مانند جدید از نقاط ایزوالکتریک کم از قارچ شیتاکی (Lentinula edodes)
کلمات کلیدی
پروتئین متصل به بیوتین؛ عصاره سلولی؛ نقطه ایسوالکتریک؛ لندنولا edodes؛ اتصال غیراختصاصی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• A biotin-binding protein with a low isoelectric point (pI) is potentially valuable.
• We discovered novel biotin-binding proteins (lentiavidins) from Lentinula edodes.
• The pI of lentiavidin 1 was 3.9; the lowest in the known biotin-binding proteins.
• Lentiavidin 1 has potential broad applications as a novel biotin-binding protein.

A biotin-binding protein with a low isoelectric point (pI), which minimizes electrostatic non-specific binding to substances other than biotin, is potentially valuable. To obtain such a protein, we screened hundreds of mushrooms, and detected strong biotin-binding activity in the fruit bodies of Lentinula edodes, shiitake mushroom. Two cDNAs, each encoding a protein of 152 amino acids, termed lentiavidin 1 and lentiavidin 2 were cloned from L. edodes. The proteins shared sequence identities of 27%–49% with other biotin-binding proteins, and many residues that directly associate with biotin in streptavidin were conserved in lentiavidins. The pI values of lentiavidin 1 and lentiavidin 2 were 3.9 and 4.4, respectively; the former is the lowest pI of the known biotin-binding proteins. Lentiavidin 1 was expressed as a tetrameric protein with a molecular mass of 60 kDa in an insect cell-free expression system and showed biotin-binding activity. Lentiavidin 1, with its pI of 3.9, has a potential for broad applications as a novel biotin-binding protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 121, Issue 4, April 2016, Pages 420–423
نویسندگان
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