کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
20312 43168 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathway
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Inhibitory effect of fucosylated chondroitin sulfate from the sea cucumber Acaudina molpadioides on adipogenesis is dependent on Wnt/β-catenin pathway
چکیده انگلیسی

The fucosylated chondroitin sulfate was isolated from the sea cucumber Acaudina molpadioides (Am-CHS) and its effect on the adipogenesis was investigated in vitro. Results showed that Am-CHS exhibited a marked anti-adipogenic effect. The TG contents of cells treated with Am-CHS from the early stage, mid stage and later stage were decreased by 31.76%, 19.76% and 13.85%, respectively. Wnt/β-catenin pathway acts as a negative regulator of adipogenesis. It is showed that Am-CHS enhanced the expression of the Wnt/β-catenin related factors, namely Wnt10b, β-catenin, Fz and LRP, causing more cytoplasmic β-catenin translocated into the nuclear to inhibit the expression of PPARγ and C/EBPα. SREBP-1c promotes the adipocyte differentiation by enhancing the activity of PPARγ. Reverse transcriptase-polymerase chain reaction assay and western blotting results revealed that the expressions of SREBP-1c and its transcriptional product FAS were inhibited by Am-CHS. Further study showed that Am-CHS also suppressed the PPARγ and C/EBPα expression. These results indicate that the anti-adipogenic activity of Am-CHS may exhibited by activating the Wnt/β-catenin pathway and down-regulating the SREBP-1c expression. To further verify this conclusion, in vivo experiments were performed. Results revealed that Am-CHS reduced the body adipose rate compared to high-fat-high sucrose diet (HFSD)-fed mice. Western blotting results showed that Am-CHS increased the expression of β-catenin, a negative regulator in adipogenesis, and decreased that of SREBP-1c, PPARγ and C/EBPα, the positive regulators of adipogenesis in the adipose tissue compared to the HFSD-fed group. These results further confirmed the anti-adipogenic activity of Am-CHS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 1, January 2015, Pages 85–91
نویسندگان
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