کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5132993 | 1492053 | 2018 | 9 صفحه PDF | دانلود رایگان |
- A novel glycine-rich antioxidant peptide purified from Bacillus amyloliquefaciens.
- Showed antioxidant potential via Nrf-2 mediated heme oxygenase-1 expression.
- YD1 displayed active against gram-positive, negative and resistant bacteria.
A novel antioxidant peptide YD1 (â¼1.0Â kDa), purified from the strain Bacillus amyloliquefaciens CBSYD1, displayed activity in several in vitro assays and was also efficient against Gram-positive, Gram-negative as well as multidrug-resistant (MDR) bacteria. Strain growth was adapted to bile-salt conditions where a clear halos-zone was observed in a bile-salt plate assay and was viable in different digestive track conditions. YD1 treatment on RAW 264.7 cells increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) through the enhanced levels of heme oxygenase-1 (HO-1). Furthermore, the YD1-treated group showed higher levels of antioxidant enzymes compared to the oxidative stress group. YD1 demonstrated a strong antioxidant activity by decreasing nitric oxide (NO) and reactive oxygen species (ROS) in RAW 264.7 cells. This study suggests that YD1 and the strain could be a natural antioxidant and a probiotic candidate respectively.
Journal: Food Chemistry - Volume 239, 15 January 2018, Pages 502-510