کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7557087 | 1491306 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fullerene-PAMAM(G5) composite modified impedimetric biosensor to detect Fetuin-A in real blood samples
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study is to develop a nanomaterial-dendrimer composite modified biosensor to detect Fetuin-A (HFA) in real blood samples. For this purpose, we designed an Electrochemical Impedance Spectroscopy (EIS) based anti-Fetuin-A (Anti-HFA) modified biosensor system and tested in real blood samples. Chronoimpedance was also employed. The same samples were analyzed with ELISA and the results were compared for validation of the new system. Gold screen printed electrodes (AuSPE) were used as transducer. Firstly, a self-assembly monolayer (SAM) was formed on gold surface by 4-aminothiophenol (4-ATP), Fullerene and PAMAM-NH2 (G5), layers were formed, consecutively. Then Anti-HFA was immobilized on Au/4-ATP/Fullerene/PAMAM electrode via glutaraldehyde. The chronoimpedance test was employed to investigate the optimum analysis duration. According to the data of chronoimpedance, the total analysis time for EIS was chosen as 3Â min. The new biosensor was compared with the ELISA method which required 150Â min. The calibration curve was prepared electron transfer resistance of the electrode (ÎRet) per minute as ohm and 1.66-134Â ng/mL.min with a R2Â =Â 0.9912. The LOD and LOQ of the biosensor was calculated as 0.48Â ng/mL.min, 1.46Â ng/mL.min, respectively. Linear regression analysis indicated that the novel developed biosensor results agreed well with that of the conventional ELISA assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 542, 1 February 2018, Pages 11-15
Journal: Analytical Biochemistry - Volume 542, 1 February 2018, Pages 11-15
نویسندگان
Zihni Onur Uygun, ÃaÄdaÅ Åahin, Merve Yılmaz, Yasemin Akçay, Ali Akdemir, Ferhan SaÄın,