Effect of Commiphora mukul gum resin on hepatic and renal marker enzymes, lipid peroxidation and antioxidants status in pancreas and heart in fructose fed insulin resistant rats

This work aims to study the antioxidant efficacy of Commiphora mukul (C. mukul) gum resin ethanolic extract in high fructose diet (HFD) insulin resistant rats. The male Wistar albino rats were randomly divided into four groups of eight animals each; two of these groups (Control group [C] and Control treated with C. mukul [C + CM]) were fed with standard pellet diet and the other two groups (Fructose fed rats [F-group] and fructose fed with C. mukul treated group [F + CM]) were fed with high fructose diet (HFD) (66%). C. mukul gum resin ethanolic extract (200 mg/kg body weight/day) was administered orally to group C + CM and group F + CM. At the end of 60-day experimental period biochemical parameters related to antioxidant, oxidative stress marker enzymes and hepatic and renal marker enzymes of tissues were performed. The fructose fed rats showed increased level of enzymatic activities aspartate aminotransminases (AST), alanine aminotransminases (ALT) in liver and kidney and oxidative markers like lipid peroxidation (LPO) and protein oxidation (PO) in pancreas and heart. Antioxidant enzyme activities were significantly decreased in the pancreas and heart compared to control groups. Administration of C. mukul (200 mg/kg bwt) to fructose fed insulin resistant rats for 60 days significantly reversed the above parameters toward normal. In conclusion, our data indicate the preventive role of C. mukul against fructose-induced insulin resistance and oxidative stress; hence this plant could be used as an adjuvant therapy for the prevention and/or management of chronic diseases characterized by hyperinsulinemia, insulin resistance and aggravated antioxidant status.