کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9603203 | 43279 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Existence of an iron-oxidizing bacterium Acidithiobacillus ferrooxidans resistant to organomercurial compounds
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کلمات کلیدی
Hg0MMCEMCATCCAcidithiobacillus ferrooxidanspCMBp-chloromercuribenzoic acidHg2+ - Hg2 +PMA - LDC هاIron-oxidizing bacterium - باکتری اکسید کننده آهنMercuric reductase - ردوکتاز مروجیکAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییMethylmercury chloride - کلرید متیل کرچکMercuric ion - یون Mercuric
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Acidithiobacillus ferrooxidans MON-1 which is highly resistant to Hg2+ could grow in a ferrous sulfate medium (pH 2.5) with 0.1μM p-chloromercuribenzoic acid (PCMB) with a lag time of 2 d. In contrast, A. ferrooxidans AP19-3 which is sensitive to Hg2+ did not grow in the medium. Nine strains of A. ferrooxidans, including seven strains of the American Type Culture Collection grew in the medium with a lag time ranging from 5 to 12 d. The resting cells of MON-1, which has NADPH-dependent mercuric reductase activity, could volatilize Hg0 when incubated in acidic water (pH 3.0) containing 0.1 μM PCMB. However, the resting cells of AP19-3, which has a similar level of NADPH-dependent mercuric reductase activity compared with MON-1, did not volatilize Hg0 from the reaction mixture with 0.1 μM PCMB. The activity level of the 11 strains of A. ferrooxidans to volatilize Hg0 from PCMB corresponded well with the level of growth inhibition by PCMB observed in the growth experiments. The resting cells of MON-1 volatilized Hg0 from phenylmercury acetate (PMA) and methylmercury chloride (MMC) as well as PCMB. The cytosol prepared from MON-1 could volatilize Hg0 from PCMB (0.015 nmol mgâ1 hâ1), PMA (0.33 nmol mgâ1 hâ1) and MMC (0.005 nmol mgâ1 hâ1) in the presence of NADPH and β-mercaptoethanol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 99, Issue 6, June 2005, Pages 586-591
Journal: Journal of Bioscience and Bioengineering - Volume 99, Issue 6, June 2005, Pages 586-591
نویسندگان
Fumiaki Takeuchi, Atsunori Negishi, Sosaku Nakamura, Tadayoshi Kanao, Kazuo Kamimura, Tsuyoshi Sugio,