کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1365852 981575 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and application of 2′-fluoro-substituted cap analogs
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Synthesis and application of 2′-fluoro-substituted cap analogs
چکیده انگلیسی

Design, synthesis, and biological evaluation of 2′-fluoro-substituted cap analogs, i.e., m7,2′FG[5′]ppp[5′]G and m7,2′FG[5′]ppp[5′]m7G are described. Structures were confirmed by 1H, 31P, 19F NMR and MS data. The effects of the 2′-fluoro-substituted moiety from the normal and N7 double methylated mCAP were evaluated with respect to their capping efficiency, in vitro T7 RNA polymerase transcription efficiency, and translation activity using cultured HeLa cells. Luciferase fusion protein production was monitored by measuring the luciferase activity. mRNA poly(A) capped with 2′-fluoro-substituted cap analogs, (m7,2′FG[5′]ppp[5′]G) and (m7,2′FG[5′]ppp[5′]m7G), were translated ∼2.4- and 2.5-fold more efficiently, respectively, than mRNA capped with conventional m7G[5′]ppp[5′]G.

Design, synthesis, and biological evaluation of 2′-fluoro-substituted cap analogs i.e., m7,2′FG[5′]ppp[5′]G and m7,2′FG[5′]ppp[5′]m7G are reported.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 17, Issue 19, 1 October 2007, Pages 5295–5299
نویسندگان
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