Bioactive minor components of the total salvianolic acids injection prepared from Salvia miltiorrhiza Bge.

The total salvianolic acids are main effective constituents of Salvia miltiorrhiza Bge., a traditional Chinese medicine used for thousands of years. The purpose of present study was to make clear the composition and bioactivities of the minor components of the total salvianolic acids injection. As a result, three new minor phenolic acids (1–3) together with six known compounds (4–9) were characterized from the total salvianolic acids injection. Their structures were elucidated by extensive analysis of the spectral data. The absolute configuration of compounds 1–3 were confirmed by their J7′,8′ observed in 1H NMR spectra, absorption band at approximately 250–260 nm in their CD spectra as well as chemical shifts of C-8″ and C-8‴ displayed in 13C NMR spectra. Then DPPH free radical scavenging assay and NAD(P)H:quinine oxidoreductase 1 (NQO1) inducing activity test were employed to evaluate the antioxidant effect of new minor compounds 1 and 2. Compound 2 showed significant NQO1 inducing activity at 20 μM with IR value 2.6. Meanwhile, DPPH scavenging assay revealed that the inhibition rates of compounds 1 and 2 were 84.3% and 74.9% at 2 mM, respectively.

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