کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10133604 | 1645599 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro selection of DNA aptamers for the development of fluorescent aptasensor for sarcosine detection
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to develop a simple, convenient and economic method to detect and quantify the level of sarcosine in human urine samples, we have selected DNA aptamers for sarcosine through a modified affinity chromatography SELEX (Systematic Evolution of Ligands by Exponential Enrichment), which is based on the aptamer sequences bound by the target molecules would be released from the DNA hybridizations coupled on streptavidin-agarose beads. 4 aptamer candidates were obtained after 12 rounds of selection, and their affinities were preliminarily checked by SYBR green I staining. The best of them is Sar11, which with the dissociation constant (Kd) of 134.8ânM was further modified and used to design a fluorescence sensor to quantify sarcosine. The detection limit of sarcosine by this sensor is 55ânM, while its quantitative range is 100 to 2000ânM. Also, the aptamer Sar11 shows good selectivity for sarcosine to its analogues. Moreover, the fluorescent aptasensor obtains a satisfying result for the detection of sarcosine in human urine samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 276, 10 December 2018, Pages 128-135
Journal: Sensors and Actuators B: Chemical - Volume 276, 10 December 2018, Pages 128-135
نویسندگان
Yu Luo, Jine Wang, Luyan Yang, Tian Gao, Renjun Pei,