کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10226375 | 1701264 | 2019 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A simple electrochemical immunosensor platform for detection of Apolipoprotein A1 (Apo-A1) as a bladder cancer biomarker in urine
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A simple electrochemical immunosensor platform for detection of Apolipoprotein A1 (Apo-A1) as a bladder cancer biomarker in urine A simple electrochemical immunosensor platform for detection of Apolipoprotein A1 (Apo-A1) as a bladder cancer biomarker in urine](/preview/png/10226375.png)
چکیده انگلیسی
Bladder cancer is one of the tumors associated with the highest mortality rate. It is clinically characterized by high recurrent rates and poor prognosis once tumors invade the lamina propia. Nevertheless, current diagnostic techniques such as cystoscopy and cytology are invasive, time-consuming, and expensive. To address these challenges, we developed an electrochemical enzyme-linked immunosorbent assay (eELISA) platform on indium tin oxide (ITO) glass strips to quantify Apolipoprotein-A1 (Apo-A1) proteins in urine reported as an early bladder cancer biomarker with high sensitivity and specificity. We thoroughly studied the kinetics of ALP enzyme on the surface of ITO electrode so that we determined the minimum incubation time (2âmin) and temperature (25â°C) for electrochemical measurement. Combined with chronocoulometry (CC) method under optimized conditions, our developed ITO-based eELISA platform can sensitively detect the existence of Apo-A1 as low as 1âpM in both PBS and urine with the dynamic range of concentrations (1âpM-100ânM) in a small one-drop assay system. Also, the simply prepared detection platform was successfully applied to urine condition. By using the developed platform, urine samples of bladder patients were clearly distinguished from normal controls. Our developed eELISA platform offers a simple and sensitive immunoassay approach for point-of-care (POC) diagnostic system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 278, 1 January 2019, Pages 103-109
Journal: Sensors and Actuators B: Chemical - Volume 278, 1 January 2019, Pages 103-109
نویسندگان
Seong-Eun Kim, Young Joo Kim, Sunga Song, Kook-Nyung Lee, Woo Kyeong Seong,