Metabolomics-assisted synthetic biology

► Non-targeted tracer fate detection (NTFD) adds new knowledge and a new dimension to metabolomics and synthetic bioprocesses. ► How multiple functional analyses can be integrated to help understand carbohydrate utilisation. ► The importance of biofuel production illustrated via the engineering of aquatic cyanobacteria. ► TARDIS-based mass isotopomer analysis for microbial metabolomics. ► Reverse engineering and inference of metabolic networks using computational approaches.