Metabolic flux rewiring in mammalian cell cultures

- Proliferating mammalian cultures exhibit 'wasteful' glucose and glutamine metabolism.
- Stationary cultures shift to oxidative phosphorylation and reduce lactate production.
- Metabolism is regulated dynamically by expression and activation of enzymes.
- Metabolic flux analysis provides a global picture of dynamic flux rewiring.
- Metabolic engineering can be applied to redirect cells toward desired phenotypes.