Harnessing glycosylation to improve cellulase activity

► Glycosylation affects cellulase activity in plant cell wall deconstruction. ► Glycosylation pathways can be manipulated for probing glycan function. ► Better characterization of glycosylation-trimming enzymes is needed. ► Glycosylation may play additional functional roles beyond proteolysis protection. ► Computational, genomic, and biophysical tools are needed to probe glycosylation.