Photolytic oxidation of Coomassie Brilliant Blue with H2O2

Coomassie Brilliant Blue, a biochemically important dye, was subjected to UV radiation in the presence of hydrogen peroxide. The photo-oxidation of the dye was monitored spectrophotometrically. The rate of decolorisation and degradation were calculated from the observed data and were found to be first order. A plausible explanation involving the probable radical initiated mechanism was given to explain this behaviour.