Modeling the liquid filling in capillary well microplates for analyte preconcentration

► Capillary microplate wells with hydrophobic surfaces offers preconcentration with self-filling. ► Simulations show that the gravitational effect cannot be ignored for high liquid volumes. ► A modifying equation from a series of simulation results accounted for the gravitational effect. ► The presence of multiple holes under the droplet did not affect the simulation outcomes much.