Spectroscopic determination of Critical Micelle Concentration in aqueous and non-aqueous media using a non-invasive method

► CMC of TX-100 determined by non-invasive methodology. ► Micellar and Reverse Micellar systems characterized. ► Structural perturbations induced by external fluorophores avoided. ► Steady-state and time-resolved measurements are highly correlated. ► Three external probes used also support the non-invasive technique.