How to obtain a well-spread monolayer of lysozyme at the air/water interfaces

► The working conditions for a full spreading of lysozyme monolayer were studied. ► Trurnit's spreading method, ionic strength substrate, spread number of molecules and waiting time for the recorded of the monolayer were analyzed. ► The limiting area corresponding to a complete spreading of unfolding lysozyme monolayer was 1 m2/mg.